Composite

Part:BBa_K3700004

Designed by: Juliette Moreau   Group: iGEM20_Montpellier   (2020-10-14)


dacA+sfGFP with pTAC promoter +features

This part contains sfGFP linked to the dacA gene with the promoter pTAC and the rbs J61101. There is the 10 AA linker K105012 to avoid any steric hindrance between the two proteins. All is surrounded by spacers (0, 3, 4 and 5) in order to create universal primers and terminators in order to assure the good stop of translation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1643
    Illegal BamHI site found at 1809
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1669
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 871


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