![](https://parts.igem.org/images/partbypart/icon_device.png)
Device
Part:BBa_K3694026
Designed by: Luke James Gerassimou Group: iGEM20_DeNovocastrians (2020-10-21)
Biosensor construct (Theoretical)
Biosensor gene construct that would work in E.coli. The construct contains transcription factor CaTM and its binding site connected to a downstream fluorescent mCherry gene. The transcription factor, in the presence of its ligand catechol or cis,cis-Muconate, would bind its binding region to recruit RNAP and transcribe the downstream fluorescent protein gene. The construct also contains benM transcription factor and its binding region which is connected to a downstream green fluorescence gene. In the presence of benzene and cis,cis-Muconate, the transcription factor binds to the binding region to recruit RNAP and transcribe the downstream fluorescent protein gene.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1610
Illegal PstI site found at 777 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 742
Illegal NheI site found at 765
Illegal NheI site found at 834
Illegal NheI site found at 857
Illegal PstI site found at 777 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 822
Illegal XhoI site found at 881 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1610
Illegal PstI site found at 777 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1610
Illegal PstI site found at 777
Illegal AgeI site found at 1037 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |