Device

Part:BBa_K3694026

Designed by: Luke James Gerassimou   Group: iGEM20_DeNovocastrians   (2020-10-21)


Biosensor construct (Theoretical)

Biosensor gene construct that would work in E.coli. The construct contains transcription factor CaTM and its binding site connected to a downstream fluorescent mCherry gene. The transcription factor, in the presence of its ligand catechol or cis,cis-Muconate, would bind its binding region to recruit RNAP and transcribe the downstream fluorescent protein gene. The construct also contains benM transcription factor and its binding region which is connected to a downstream green fluorescence gene. In the presence of benzene and cis,cis-Muconate, the transcription factor binds to the binding region to recruit RNAP and transcribe the downstream fluorescent protein gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1610
    Illegal PstI site found at 777
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 742
    Illegal NheI site found at 765
    Illegal NheI site found at 834
    Illegal NheI site found at 857
    Illegal PstI site found at 777
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 822
    Illegal XhoI site found at 881
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1610
    Illegal PstI site found at 777
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1610
    Illegal PstI site found at 777
    Illegal AgeI site found at 1037
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None