Coding

Part:BBa_K3694023

Designed by: Darcy Kilvert   Group: iGEM20_DeNovocastrians   (2020-10-21)


benABCD gene cluster

The benABCD gene cluster encodes for the benzene degradation pathway that degrades benzene into catecol. The cluster encodes for a benzoate 1,2-dioxygenase (benABC) which converts benzene into Cis-1,2-dihydroxycyclohexadiene. This is converted to catecol by the benD encoded dehydrogenase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1573
    Illegal BamHI site found at 3133
    Illegal XhoI site found at 133
    Illegal XhoI site found at 529
    Illegal XhoI site found at 595
    Illegal XhoI site found at 1135
    Illegal XhoI site found at 2508
    Illegal XhoI site found at 2700
    Illegal XhoI site found at 3258
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 314
    Illegal NgoMIV site found at 415
    Illegal NgoMIV site found at 763
    Illegal NgoMIV site found at 1171
    Illegal NgoMIV site found at 1526
    Illegal NgoMIV site found at 2574
    Illegal NgoMIV site found at 2903
    Illegal AgeI site found at 79
    Illegal AgeI site found at 1639
    Illegal AgeI site found at 1674
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3144
    Illegal BsaI.rc site found at 4085


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Categories
Parameters
None