Coding
Part:BBa_K3694023
Designed by: Darcy Kilvert Group: iGEM20_DeNovocastrians (2020-10-21)
benABCD gene cluster
The benABCD gene cluster encodes for the benzene degradation pathway that degrades benzene into catecol. The cluster encodes for a benzoate 1,2-dioxygenase (benABC) which converts benzene into Cis-1,2-dihydroxycyclohexadiene. This is converted to catecol by the benD encoded dehydrogenase.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1573
Illegal BamHI site found at 3133
Illegal XhoI site found at 133
Illegal XhoI site found at 529
Illegal XhoI site found at 595
Illegal XhoI site found at 1135
Illegal XhoI site found at 2508
Illegal XhoI site found at 2700
Illegal XhoI site found at 3258 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 314
Illegal NgoMIV site found at 415
Illegal NgoMIV site found at 763
Illegal NgoMIV site found at 1171
Illegal NgoMIV site found at 1526
Illegal NgoMIV site found at 2574
Illegal NgoMIV site found at 2903
Illegal AgeI site found at 79
Illegal AgeI site found at 1639
Illegal AgeI site found at 1674 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3144
Illegal BsaI.rc site found at 4085
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Categories
Parameters
None |