Composite

Part:BBa_K3693005

Designed by: Jie Gao   Group: iGEM20_BJ101HS   (2020-10-19)


For secreting the lactoferrin outside yeast

The composite part consists the a secretion signal E.coli that can trigger the expression of the protein exerted in, and the sequence of LTF we exerted in. This sequence enables the bacteria produce lactotransferrin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 67
    Illegal BglII site found at 829
    Illegal BglII site found at 1384
    Illegal BglII site found at 1555
    Illegal BglII site found at 1816
    Illegal XhoI site found at 2380
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 583
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 279
    Illegal BsaI.rc site found at 453
    Illegal SapI.rc site found at 1079


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Categories
Parameters
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