Part:BBa_K3692000

DGA1

Usage and Biology

DGA1 is a yeast gene which codes for transmembrane protein diacylglycerol acyltransferase. This protein is responsible for the catalysis of the terminal step of TAG formation: the formation of TAG from DAG. Further, TAGs constitute the core structure of LDs. Thus, DGA1 plays an important role in LD biogenesis.

The expression of DGA1 is tightly regulated on the transcriptional level. It is influenced by growth conditions such as cell density and nitrogen deprivation. Experiments have shown that DGA1 can mediate essentially all cellular TAG synthesis in the stationary phase (Lewin et al., 1999).

It has been observed that the overexpression of DGA1 from GAL1 promoter results in 30% increase of the lipid content in S. cerevisiae (Polburee et al., 2018).

Dga1 is localized in the ER membrane, but it lacks a classical signal peptide (Lewin et al., 1999).

References

• Lewin, T. M., Wang, P., & Coleman, R. A. (1999). Analysis of amino acid motifs diagnostic for the sn-glycerol-3-phosphate acyltransferase reaction. Biochemistry, 38(18), 5764–5771. https://doi.org/10.1021/bi982805d
• Polburee, P., Ohashi, T., Tsai, Y. Y., Sumyai, T., Lertwattanasakul, N., Limtong, S., & Fujiyama, K. (2018). Molecular cloning and overexpression of DGA1, an acyl-CoA-dependent diacylglycerol acyltransferase, in the oleaginous yeast rhodosporidiobolus fluvialis DMKU-RK253. Microbiology (United Kingdom), 164(1), 1–10. https://doi.org/10.1099/mic.0.000584

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]