Part:BBa_K3684002
Rat Diamine Oxidase
Diamine Oxidases, DAO, are widespread among living organisms and found in both microorganisms and in plants and animals. DAO is responsible for histamine metabolism: it was also named histaminase because it was identified as the enzyme that cleared histamine from minced lung and liver samples. DAO was proposed to represent a safeguard against deleterious effects from exogenous amines. For example, it is related to histamine detoxification in the intestinal tract and to putrescine and histamine detoxification in placental tissues. And rat Diamine Oxidase, rDAO, plays an important role in oxidizing histamine in rat. This protein can be expressed and purified to degrade histamine.
Contribution: HKUST 2022
Team HKUST 2022 add a N-terminal pelB leader sequence to rDAO. Adding pelB tag to rDAO increases the functional expression of rDAO in our DH5α chassis. To know more about our experiment result, refer to composite parts BBa_K4225022 and BBa_K4225007, while to know more about effect on pelB on rDAO in our DH5α chassis, refer to our wiki page.
Contribution: TERMOSZ-Selye-HUN 2024
Team TERMOSZ-Selye-HUN 2024 took further codon optimisation for the e.coli chassis, and experimented to evaluate whether this had impacted the production or the function of the protein. We have utilised the E. coli strain DH10B for our experiments. Refer to parts BBa_K5492200, BBa_K5492203 and our team wiki here.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1004
Illegal BamHI site found at 1932
Illegal BamHI site found at 2186
Illegal XhoI site found at 802 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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