Coding

Part:BBa_K3682007

Designed by: YOU-CHENG LEE   Group: iGEM20_NYMU-Taipei   (2020-10-15)


cleavage site 4 on spike protein sequence

this sequence will be translated to a polypeptide which is part of the SARS CoV2 spike protein and includes one of the four cleavage sites for pepP cleavage. We constructed this sequence because pepP may not cut the whole spike protein due to its huge structure. This sequence includes the cutting site sequence and a few sequences upstream and downstream (upstream 33 and downstream 30)

Usage and Biology

One of the cleavage site pepP protease will identify is Phe-Pro amino acid sequence. After the cleavage of pepP, the polypeptide may be cut into two smaller parts with 1.16kDa and 1.30kDa each.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None