DNA

Part:BBa_K3682003

Designed by: Yu-Fan,Lin   Group: iGEM20_NYMU-Taipei   (2020-10-15)


RFP sequence

This sequence includes mRFP coding sequence, a TEV cleavage site, and a 6x histag. This part has two functions for our group. One is to check if the polyHEMA gel we are working on can chelate his tagged proteins as we designed, another is to serve as the part we are aiming to meet the criteria "improvement of an existing part."


Fig1. RFP sequence with TEV cleavage site and 6xhistag overview


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None