Coding

Part:BBa_K3682002

Designed by: YOU-CHENG LEE   Group: iGEM20_NYMU-Taipei   (2020-10-15)

hACE2 sequence

This part conatins the sequence of human ACE2 (Angiotensin-converting enzyme 2) receptor binding domain(residues 19-615), which is the receptor SARS CoV2 spike protein binds to in the human body. There is also a linker originally on ACE2 in this sequence(residues 616-740), we retained this sequence because we are going to stick our construct to a gel surface, so a linker creating distance between two parts may be useful to retain ACE2's structure instead of being interrupted by the gel. We also added a TEV cleavage site and a 6xhistag behind its sequence for later protein purification.


Fig1. Structure of ACE2 RBD and its natural linker.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1800
    Illegal PstI site found at 289
    Illegal PstI site found at 1232
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1800
    Illegal PstI site found at 289
    Illegal PstI site found at 1232
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1800
    Illegal BglII site found at 505
    Illegal BamHI site found at 1070
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1800
    Illegal PstI site found at 289
    Illegal PstI site found at 1232
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1800
    Illegal PstI site found at 289
    Illegal PstI site found at 1232
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 8
    Illegal SapI site found at 295


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Categories
Parameters
None