Part:BBa_K3682001

pepP sequence

this sequence will be translated into protease pepP, which we use to try to cleave SARS CoV2 spike protein and its fragments in our project. We constructed a searching software to look for suitable proteases that may cut the corresponding sequence on SARS CoV2 spike protein or its fragments on MEROPS, which is a protease database.

Usage and Biology

According to MEROPS’s data, PepP has four cleavage sites on spike protein(326 ~ 333 IVRFPNIT, 487 ~ 494 NCYFPLQS, 1049 ~ 1056 LMSFPQSA, 1086 ~ 1093 KAHFPREG, between F&P). However, pepP left a cleavage site on hACE2’s RBD(protein sequence 364~371 DMAYAAQP, between Y&A), which could lead to further malfunction problem. As a result, we created a point mutation on hACE2, changing sequence YAA into AAA to prevent protease reaction. This design ensures the fusion protein intact and function normally.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 260
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1243
  
• 1000
  COMPATIBLE WITH RFC[1000]