Coding

Part:BBa_K368012

Designed by: Jara Radeck   Group: iGEM10_LMU-Munich   (2010-10-24)


CMV-promoter+TEV-protease+p14*+TEV recogn. site+eGFP+SV40PA

eukaryots: CMV-Promoter: konstitutive promoter from cucumber mosaic virus TEV-Protease: Protesase which cuts at the end of specific recognition sites p14*: Protein interacting with SF3b155 (Splicing factor 3b, size: 155kDa) TEV recogn. site: recognition site for TEV-protease eGFP: eukaryotic codon optimized green fluorescent protein SV40PA:polyadenylation signal, needed for translation in eukaryots

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2856
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1493


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Categories
Parameters
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