Coding

Part:BBa_K368009

Designed by: Jara Radeck   Group: iGEM10_LMU-Munich   (2010-10-24)


tet-on Promoter+TEV recogn.site+N-degron+SF3b155+Human Bak+SV40PA

eukaryots: tet-on Promoter: doxycycline inducible CMV promoter (special cell line needed!) TEV recogn.site: specific restriction site for TEV protease N-degron: degradation signal for proteins if N-terminus is free SF3b155: Splicing factor 3b, size: 155kDa, interacting with p14* Human Bak: mitochondrial membrane protein, causing apoptosis if overexpressed SV40PA: polyadenylation signal, needed for translation in eukaryots

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
    Illegal XhoI site found at 2480
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2029
    Illegal SapI.rc site found at 1201


[edit]
Categories
Parameters
None