Intermediate

Part:BBa_K368005

Designed by: Jara Radeck   Group: iGEM10_LMU-Munich   (2010-10-20)


PhiC31o Integrase + SV40 Polyadenylation site

This vector contains a PhiC31o Integrase and a SV40-Polyadenylation site for expression in eucaryotic cells. Without promoter!


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 109
    Illegal PstI site found at 475
    Illegal PstI site found at 1012
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 109
    Illegal PstI site found at 475
    Illegal PstI site found at 1012
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2042
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 109
    Illegal PstI site found at 475
    Illegal PstI site found at 1012
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 109
    Illegal PstI site found at 475
    Illegal PstI site found at 1012
    Illegal NgoMIV site found at 238
    Illegal NgoMIV site found at 859
    Illegal AgeI site found at 1154
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 201
    Illegal BsaI.rc site found at 538
    Illegal BsaI.rc site found at 781
    Illegal BsaI.rc site found at 941
    Illegal BsaI.rc site found at 957
    Illegal BsaI.rc site found at 1294
    Illegal SapI.rc site found at 444
    Illegal SapI.rc site found at 1356


[edit]
Categories
Parameters
None