Composite

Part:BBa_K3675005

Designed by: Tiago Lopes   Group: iGEM20_NOVA_LxPortugal   (2020-10-22)


Prom2_GFP_T1

This composite part has an improved GFP coding gene (BBa_K3675002) with its expression controlled by a Pseudomonas putida regulatory region (BBa_K3675001) upstream. A strong terminator is inserted downstream to ensure that there is no leaky signal (BBa_K3675003) This part can be therefore used to detect the presence of alpha-pinene in medium when transformed in a Pseudomonas putida strain capable of degrading alpha-pinene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 607
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 676
    Illegal PstI site found at 607
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1195
    Illegal XhoI site found at 84
    Illegal XhoI site found at 1096
    Illegal XhoI site found at 1410
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 607
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 607
    Illegal NgoMIV site found at 1509
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1500
    Illegal SapI site found at 314
    Illegal SapI site found at 1457


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Categories
Parameters
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