Composite

Part:BBa_K3675004

Designed by: João Costa   Group: iGEM20_NOVA_LxPortugal   (2020-10-22)


Prom1_GFP_T1

This composite part has an improved GFP coding gene (BBa_K3675002) with its expression controlled by a Pseudomonas rhodesiae regulatory region (BBa_K3675000) upstream. A strong terminator is inserted downstream to ensure that there is no leaky signal (BBa_K3675003) This part can be therefore used to detect the presence of alpha-pinene in the medium when transformed in a Pseudomonas putida strain capable of degrading alpha-pinene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 64
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 583
    Illegal XhoI site found at 484
    Illegal XhoI site found at 798
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 897
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 888
    Illegal SapI site found at 845


[edit]
Categories
Parameters
None