Regulatory

Part:BBa_K3675000

Designed by: Tiago Lopes   Group: iGEM20_NOVA_LxPortugal   (2020-10-22)


alpha pinene oxide lyase promoter

The alpha-Pinene oxide is an intermediate in the degradation of alpha-pinene(an organic compound of the terpene class, one of two isomers of pinene) that is an intermediate that is cleaved by a lyase in Pseudomonas rhodesiae. This lyase is coded by the gene where this promoter belongs. The gene expression is induced indirectly by the compound alpha-Pinene, so for this part we isolated the promotor of this gene in order to obtain promoter that will allow the expression of our genes of interest by the presence of alpha-Pinene. This promotor should only be used in Pseudomonas putida since this organism have the pathway for alpha-Pinene degradation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None