Part:BBa_K3670001
algD promoter with luxI and luxR followed by mCherry
This is essentially a composite part that involves the algD promoter, LuxI and LuxR, and mCherry. All of these parts complement each other and characterize the system to be a bio-detector for RDX, a nitro-explosive. The algD promoter is an inducible promoter that activates under the presence of RDX. Activation of this promoter gives way to the gene expression of LuxI and LuxR. The LuxI gene produces a synthase that converts SAM into acyl-homoserine lactone (AHL), which can diffuse across cell membranes, allowing for quorum sensing to take place. In the case of LuxR, when bound to AHL, it produces a protein that can stimulate transcription from the right-hand lux promoter (LuxpR). mCherry works as a reporter gene for this device.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 646
Illegal SpeI site found at 399 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 646
Illegal SpeI site found at 399 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 646
Illegal BglII site found at 1544 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 646
Illegal SpeI site found at 399 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 646
Illegal SpeI site found at 399 - 1000COMPATIBLE WITH RFC[1000]
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