Coding

Part:BBa_K3669004

Designed by: Paul Osuna-Kleist   Group: iGEM20_UC_Davis   (2020-10-27)


amdS acetamide selectable marker

amdS is a dominant selective gene marker. Gene allows organism to use acetamide as a carbon/nitrogen source for resource acquisition. Can be used in conjunction with acetamide selective media.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 170
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 225
    Illegal PstI site found at 170
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 174
    Illegal BamHI site found at 128
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 170
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 170
    Illegal NgoMIV site found at 693
    Illegal NgoMIV site found at 1167
    Illegal AgeI site found at 1545
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None