Composite

Part:BBa_K3657035

Designed by: Jannik Berger   Group: iGEM20_Heidelberg   (2020-10-21)


RNA AUAGAU binding Pumby Protein coupled with GFP

This is a fusion protein containing AUAGAU binding Pumby Protein (BBa_K3657004) and GFP (BBa_E0040).

Usage and Biology

AUAGAU binding Pumby Protein binds specifically the AUAGAU RNA sequence(BBa_K3657015). Pumby Proteins can be designed to bind a specific RNA sequence.

GFP is a green fluorescent protein


This construct was planned to be used as the positive controle in an experiment aiming to explore the ability to use RNA as a linker between proteins. You can find out more about the planned experiment here: iGEM Heidelberg 2020.

Due to the COVID19 pandemics we did not obtain empirical data about this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 184
    Illegal XhoI site found at 224
    Illegal XhoI site found at 449
    Illegal XhoI site found at 634
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1337


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Categories
Parameters
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