Part:BBa_K3657022

RNA Part of an RNA·DNA-DNA triple helix

It was proved by Kunkler et al. that the transcript of this sequence creates a triple helix with the DNA part (BBa_K3657023) in vitro. (Kunkler et al., 2019)

More information available here: iGEM Heidelberg 2020

Usage and Biology

Triple helices are one of the alternative structures formed by nucleic acids. In addition to the Watson-Crick interactions between base pairs, Hoogsteen interactions are crucial for the formation of the triple helix. The binding is sequence specific.

Following RNA parts were designed to include this triple helix forming sequence: BBa_K3657025, BBa_K3657052.

References

Kunkler CN, Hulewicz JP, Hickman SC, Wang MC, McCown PJ, Brown JA. Stability of an RNA•DNA-DNA triple helix depends on base triplet composition and length of the RNA third strand. Nucleic Acids Res. 2019; 47(14):7213-7222.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]