Composite

Part:BBa_K3657021

Designed by: Jannik Berger   Group: iGEM20_Heidelberg   (2020-10-21)


RNA Motif GUAGAG targeted PPR - Ribonuclease A Complex

This part is a fusion protein. It consists of a PPR complex that binds GUAGAG RNA loop and the Ribonuclease A.


Usage and Biology

Ribonuclease A (BBa_K3657019) is a unspecific highly active endonuclease.

Single PPR subunits bind to specific ribonucleotides. Concatenation of the subunits forms a protein that binds a specific RNA sequence.

In that way this construct can deliver Ribonuclease A to a specific RNA. It was planned to be used to bind to the following Broccoli modifications: BBa_K3657017, BBa_K3657018. More information can be found here: iGEM Heidelberg 2020.

Due to the COVID pandemic we were not able to experimentally examine performance of this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None