Coding

Part:BBa_K3657010

Designed by: Jannik Berger   Group: iGEM20_Heidelberg   (2020-10-21)


N-Terminal Cap for Pentatricopeptide Proteins

This N-terminal cab can be added when designing a PPR Protein. Together with the Solubility Helix for Pentatricopeptide Proteins (BBa_K3657011) it is used, to increase the solubility of the PPR Proteins. It can be combined with the different PPR Domains:

Adenine:BBa_K3657006

Guanine:BBa_K3657007

Uracil:BBa_K3657008

Cytosine:BBa_K3657009

Usage and Biology

PPRs are originally found in Arabidopsis thaliana . This part increases the solubility of the PPR Proteins when fused to it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None