Part:BBa_K3657002
RNA binding Pumilio Homology Domain (Uracil)
The Pumby Domains are 36 amino acid long subunits, that specifically bind one of the RNA bases. The specificity is determined by the amino acids in position 16 and 20. Multiple Pumby Domains can be assembled into one Pumby protein, which is able to bind a sequence of up to 18 ribonucleotides.
This part is the uracil binding Pumby Domain.
It can be assembled to a Pumby Protein when combined with the other Pumby Domains:
Adenine:BBa_K3657000
Guanine:BBa_K3657001
Cytosine:BBa_K3657003
See also examples of the composite parts: BBa_K3657004, BBa_K3657005)
Usage and Biology
Pumby Domains are engeneered from the Pumilio Homology Domain (Drosophila melanogaster), which is an RNA binding protein, that is made out of 6 repeating subunits containing three alpha helices and two imperfect subunits at the N- and C-Terminus. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 70
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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