Plasmid_Backbone

Part:BBa_K3653020

Designed by: Shay Bamany, Shany Greenstein, Dor Josef, Amir Betker   Group: iGEM20_Technion-Israel   (2020-10-12)


pBS1C_CotC_ACE2

This plasmid constitutes the final sequence designed for genomic integration into the amyE locus of Bacillus subtilis. This sequence allows the B.Sub to express the heterologous protein (ACE2) on the surface of the spores after sporulation thanks to the link between the coat proteins (Cot C) and the desired protein. The vector also confers Chloramphenicol antibiotic resistance to the Bacillus subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 1
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1270
    Illegal EcoRI site found at 2460
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1270
    Illegal EcoRI site found at 2460
    Illegal NheI site found at 2737
    Illegal NotI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1270
    Illegal EcoRI site found at 2460
    Illegal BglII site found at 2722
    Illegal BglII site found at 8004
    Illegal XhoI site found at 273
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 1
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1270
    Illegal EcoRI site found at 2460
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1270
    Illegal EcoRI site found at 2460
    Illegal XbaI site found at 16
    Illegal NgoMIV site found at 1931
    Illegal NgoMIV site found at 4143
    Illegal NgoMIV site found at 6469
    Illegal NgoMIV site found at 8379
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI.rc site found at 347
    Illegal SapI.rc site found at 5401


[edit]
Categories
Parameters
None