Plasmid_Backbone
pBS1C_Cot

Part:BBa_K3653018

Designed by: Shay Bamany, Shany Greenstein, Dor Josef, Amir Betker   Group: iGEM20_Technion-Israel   (2020-10-12)


pBS1C_CotC_Sybody15

This plasmid constitutes the final sequence designed for genomic integration into the amyE locus of Bacillus subtilis. This sequence allows the B.Sub to express the heterologous protein (Sybody 15) on the surface of the spores after sporulation thanks to the link between the coat proteins (Cot C) and the desired protein. The vector also confers Chloramphenicol antibiotic resistance to the Bacillus subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 1
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 535
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 535
    Illegal NheI site found at 667
    Illegal NotI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 535
    Illegal BglII site found at 652
    Illegal BglII site found at 5934
    Illegal XhoI site found at 273
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 1
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 535
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 535
    Illegal XbaI site found at 16
    Illegal NgoMIV site found at 2073
    Illegal NgoMIV site found at 4399
    Illegal NgoMIV site found at 6309
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
None