Plasmid_Backbone

Part:BBa_K3653017

Designed by: Shay Bamany, Shany Greenstein, Dor Josef, Amir Betker   Group: iGEM20_Technion-Israel   (2020-10-12)


pBS1C_CotG_ACE2

This plasmid constitutes the final sequence designed for genomic integration into the amyE locus of Bacillus subtilis. This sequence allows the B.Sub to express the heterologous protein (ACE2) on the surface of the spores after sporulation thanks to the link between the coat proteins (Cot G) and the desired protein. The vector also confers Chloramphenicol antibiotic resistance to the Bacillus subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 1
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 35
    Illegal EcoRI site found at 1667
    Illegal EcoRI site found at 2857
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 35
    Illegal EcoRI site found at 1667
    Illegal EcoRI site found at 2857
    Illegal NheI site found at 3134
    Illegal NotI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 35
    Illegal EcoRI site found at 1667
    Illegal EcoRI site found at 2857
    Illegal BglII site found at 3119
    Illegal BglII site found at 8401
    Illegal XhoI site found at 670
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 1
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 35
    Illegal EcoRI site found at 1667
    Illegal EcoRI site found at 2857
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 35
    Illegal EcoRI site found at 1667
    Illegal EcoRI site found at 2857
    Illegal XbaI site found at 16
    Illegal NgoMIV site found at 2328
    Illegal NgoMIV site found at 4540
    Illegal NgoMIV site found at 6866
    Illegal NgoMIV site found at 8776
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI.rc site found at 744
    Illegal SapI.rc site found at 5798


[edit]
Categories
Parameters
None