Part:BBa_K3653015

pBS1C_CotG_Sybody15

This plasmid constitutes the final sequence designed for genomic integration into the amyE locus of Bacillus subtilis. This sequence allows the B.Sub to express the heterologous protein (Sybody 15) on the surface of the spores after sporulation thanks to the link between the coat proteins (Cot G) and the desired protein. The vector also confers Chloramphenicol antibiotic resistance to the Bacillus subtilis.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found at 1
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 35
  Illegal EcoRI site found at 932
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1
  Illegal EcoRI site found at 35
  Illegal EcoRI site found at 932
  Illegal NheI site found at 1064
  Illegal NotI site found at 7
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1
  Illegal EcoRI site found at 35
  Illegal EcoRI site found at 932
  Illegal BglII site found at 1049
  Illegal BglII site found at 6331
  Illegal XhoI site found at 670
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 1
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 35
  Illegal EcoRI site found at 932
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 1
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 35
  Illegal EcoRI site found at 932
  Illegal XbaI site found at 16
  Illegal NgoMIV site found at 2470
  Illegal NgoMIV site found at 4796
  Illegal NgoMIV site found at 6706
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 2646
  Illegal SapI.rc site found at 3728