Part:BBa_K3653012

p416-FEC-mCore1 is a high copy number vector designed for transformation and protein expression in yeast, with URA3 as a selective marker. It has a YFP (yECitrine) gene, regulated under a strong promoter. It can also be propagated in E.coli (it has AMP antibiotic marker), hence it is a shuttle vector, however, YFP cannot be expressed in bacteria.

Angiotensin-converting enzyme 2 (ACE2) is an enzyme that is attached to the cell membranes of cells in the lungs, arteries, heart, kidneys, and intestines. It is a peptide hormone with a primary physiological role in the process of maturation of angiotensin, it controls vasoconstriction and blood pressure.

ACE2 is also the cellular receptor for SARS-CoV-2. The RBD of the S protein is recognized by the extracellular peptidase domain (PD) of ACE2 mainly through polar residues. Thus, the enzyme is “hijacked” by some coronaviruses and sets the path that allows the infection of human host cells.