Plasmid_Backbone

Part:BBa_K3653005

Designed by: Hadas Yung, Ilana Elizarov   Group: iGEM20_Technion-Israel   (2020-10-12)


p416-FEC-mCore1

p416-FEC-mCore1 is a high copy number vector designed for transformation and protein expression in yeast, with URA3 as a selective marker. It has a YFP (yECitrine) gene, regulated under a strong promoter. It can also be propagated in E.coli (it has AMP antibiotic marker), hence it is a shuttle vector, however, YFP cannot be expressed in bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3028
    Illegal PstI site found at 5593
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal PstI site found at 5593
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BamHI site found at 2795
    Illegal XhoI site found at 3769
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3028
    Illegal PstI site found at 5593
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3028
    Illegal PstI site found at 5593
    Illegal NgoMIV site found at 4312
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 3680


[edit]
Categories
Parameters
None