Plasmid_Backbone

Part:BBa_K3653004

Designed by: Ella Samuel, Shanny Ackerman   Group: iGEM20_Technion-Israel   (2020-10-11)


pET-9d

Bacterial expression vector with KAN antibiotic resistance and T7 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 585
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 229
    Illegal NheI site found at 541
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BglII site found at 643
    Illegal BamHI site found at 510
    Illegal XhoI site found at 3549
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 585
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 585
    Illegal NgoMIV site found at 675
    Illegal NgoMIV site found at 1043
    Illegal NgoMIV site found at 1203
    Illegal NgoMIV site found at 1557
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
None