T7

Part:BBa_K3653001

Designed by: Ella Samuel, Shanny Ackerman   Group: iGEM20_Technion-Israel   (2020-10-11)


T7 promoter allows over-expression of proteins in E.coli that express T7 RNA polymerase, CCTATAGTAGT

This promoter is originated from T7 bacteriophage and is recognized by T7 RNA polymerase that catalyzes the formation of RNA from DNA in the 5'→ 3' direction. While working with adaptive E.coli (that expresses the polymerase), it allows protein over-expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None