Generator

Part:BBa_K3651036

Designed by: Nathan Dayie   Group: iGEM20_Baltimore_BioCrew   (2020-10-03)


Constitutive promoter upstream of mcherry. (J23100_mcherry)

This part constitutively expresses mcherry. This part includes a constitutive promoter that is part of the Anderson family (BBa_J23100) upstream of a codon-optimized mcherry (BBa_K3651025) . In our project, we used this part as a positive control so that we could see mcherry being maximally expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None