Reporter

Part:BBa_K3651035

Designed by: Nathan Dayie   Group: iGEM20_Baltimore_BioCrew   (2020-10-10)


Iron sensitive promoter pfur23 upstream of mCherry.

This transcription unit includes an iron sensitive promoter with two Ferric Uptake Regulator (FUR) Binding site (BBa_K3651023) upstream a ribosome binding site (BBa_J61100), red fluorescence protein (BBa_K3651025), and terminator (BBa_B0010). Altogether, this is an iron sensor. When iron concentration increases (in the presence of FUR protein), red fluorescence decreases. The FUR Protein in presence of iron will block the transcription because it will bind to its FUR Box.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None