Coding

Part:BBa_K3651005

Designed by: Zoe Hsieh, Maria Lyons, Josh Dayie, Max Swann   Group: iGEM20_Baltimore_BioCrew   (2020-09-26)


Codon optimized tonB gene for Synechococcus sp. CB0101 with restriction sites removed

This construct contains the Synechocystis sp. PCC 6803 tonB gene that has been codon optimized for the CB0101 Synechocystis sp. Strain. TonB is known to be part of an energy-converting complex (with ExbB and ExbD) that allows for the active transport of vitamin B12, iron siderophores, and carbohydrates (Noinaj et al., 2010). The energy dependent uptake is found to occur in the periplasmic space of substrates such as cobalamin and iron compounds such as iron dicitrate. All of the tonB complexes are regulated by Fur and are repressed when the iron amount reaches a certain level (to prevent iron accumulation, which may produce free radicals) (Noinaj et al., 2010).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1828
    Illegal SpeI site found at 1918
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1828
    Illegal SpeI site found at 1918
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1828
    Illegal BglII site found at 2364
    Illegal BamHI site found at 2082
    Illegal XhoI site found at 2020
    Illegal XhoI site found at 2368
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1828
    Illegal SpeI site found at 1918
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1828
    Illegal SpeI site found at 1918
    Illegal NgoMIV site found at 756
    Illegal NgoMIV site found at 2504
    Illegal AgeI site found at 1537
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 214


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Categories
Parameters
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