Coding

Part:BBa_K3651003

Designed by: Max Swann, Zoe Hsieh, Maria Lyons, Josh Dayie   Group: iGEM20_Baltimore_BioCrew   (2020-09-19)


Codon-optimized ssr2333 (feoA) gene for Synechococcus sp. CB0101 with restriction sites removed

This construct contains the PCC6803 Synechocystis sp. strain ssr2333 (feoA) gene that has been codon optimized for the CB0101 Synechocystis sp. strain. It is part of the feo operon, which consists of feoABC. It is found before the feoB gene and after the fur box. It is known to be a transport gene for transition metal ion binding, along with feoB (which codes for an iron-permease protein). FeoA functions as a transition metal ion binding gene, and it may be of significance in the reductive uptake pathway, where Fe+3 is reduced and then transported as Fe+2.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None