Part:BBa_K3651002
Codon optimized idiA gene for Synechococcus sp. CB0101 with restriction sites removed
This construct contains the cyanobacteria Synechocystis sp. (strain PCC 6803 / Kazusa) futA2 (otherwise known as idiA) gene that has been codon optimized for the CB101 Synechococcus sp. Strain. The gene is likely part of a periplasmic ABC transporter complex futA1A2BC (TC 3.A.1.10.2) involved in Fe3+ ion import (ferric iron). It codes for the Iron uptake protein A2, which belongs to the bacterial solute-binding protein 1 family. It plays an important role in protecting the acceptor side of photosystem II (PSII) against oxidative damage, especially under iron-limiting growth conditions. It's also suggested to play a role (that has not yet been defined) in copper supply to thylakoid proteins. It has been documented that transcription of this gene increases when cells are grown in the absence of iron. Periplasmic levels are said to increase when cells are grown in high NaCl or in the absence of iron.1 2 3 It has also been shown that cells with this gene grow normally in the absence of iron, while cells with double knockouts of this gene and futA1 grow very poorly in the absence of iron and have a marked reduction in their ability to take up Fe3+ 2. Despite this, there is some controversy as to whether this protein preferentially binds Fe2+ or Fe3+.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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