Coding

Part:BBa_K3651001

Designed by: Zoe Hsieh, Maria Lyons, Josh Dayie, Max Swann   Group: iGEM20_Baltimore_BioCrew   (2020-09-19)


Codon optimized zupT gene for Synechococcus sp. CB0101 with restriction sites removed

This construct contains the Synechocystis sp. PCC 6803 zupT gene that has been codon optimized for the CB0101 Synechocystis sp. strain. It is known to be a Fe+2 transporter gene along with feoA and feoB, and is suggested to facilitate zinc uptake. It may also transport other divalent cations such as the ions of copper and cadmium and is said to be involved with the activity of copper ion and ferrous iron transmembrane transport (Ahlgren et al., 2019).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 550
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 501
    Illegal AgeI site found at 567
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None