Part:BBa_K3651000
Codon-optimized isiB gene (Flavodoxin) for Synechococcus sp. CB0101 with restriction sites removed
The isiB gene produces Flavodoxin in Synechocystis sp. PCC 6803. Flavodoxin is a low-potential electron donor for redox enzymes with potentials at -433 and -238 mV. It was found in high quantities by Alghren et al (Environ Microbiol, 2019). Flavodoxin shares functions with ferrodoxins, a group of proteins involved in metabolic functions, and can substitute for them when the cell is under stress. For example, during times of iron starvation, cyanobacteria ferrodoxin levels decline, and flavodoxin is induced and replaces it. Research has demonstrated though that under low-iron conditions that are sufficient to induce flavodoxin, isiB is "not essential" for organism survival (Kutzki, FEMS Microbiol Lett, 1998).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 423
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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