Coding
(L-C)3

Part:BBa_K365014

Designed by: RENAULT Renaud   Group: iGEM10_ESBS-Strasbourg   (2010-10-10)

ClpX trimer with built-in linker at N-ter end

It is basically the same assembly as BBa_K365013, but with an additional 20 aa linker (BBa_K365005) fused at N-terminal end. This part can be directly used by those who wants to fuse something in N-ter of the ClpX trimer, but one can also use a linker of his own, starting from BBa_K365013.

Background:

This construct was the final step for the ClpX hexamer creation. The two trimers will assemble to the full ClpX unit. Then the phytochrome B was cloned to this trimer. It will also be possible to add any other activation system like hormone receptors to this biobrick instead of phytochrome B, giving this part a huge potential of further applications.


You can visit our wiki "visual description" page for more info


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 765
    Illegal BglII site found at 1929
    Illegal BglII site found at 3093
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Conception:

The standards cloning methods were used. This includes digestion of the vector containing ClpX trimer and the linker part then the ligation of them and to finish the transformation of competente cells with this plasmid.

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Categories
Parameters
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