Coding
GFPsf

Part:BBa_K365009

Designed by: RENAULT Renaud   Group: iGEM10_ESBS-Strasbourg   (2010-10-10)

GFP superfolder RFC 25



Background

The GFP super fold protein originated from the BBa_I746915. This old biobrick contains the normal standard prefix and suffix. When this GFP is now fused to another biobrick a stop codon is created which would terminate the transcription of the fused biobrick. To avoid this inconvenience, we improved this biobrick to the fusion standard BBF RFC 25. This new biobrick can now be cloned to any biobrick with compatible restriction sides without creating a stop codon.


You can visit our wiki "visual description" page for more info



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13


Conception

The standard PCR technique was used. The Primer for amplification contained the fusion pre- and suffix and compatible base pair for the GFP gene.

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