Coding
λOtag

Part:BBa_K365008

Designed by: ZIEGLER Thea   Group: iGEM10_ESBS-Strasbourg   (2010-10-10)

Lambda tag

Lambda tag from E. coli. This tag has to be fused to the N-terminal end only (there is NO fusion prefix) of any given protein. The tagged protein are degraded by ClpXP only if they are tethered to it. Lambda tag is thus equivalent to DAS tag (see K365007) exept for its position within the fusion protein you will get.

Background:

The λO- tag is the N-terminal equalent to the DAS tag. Degradation of proteins bearing the N-terminal λO- tag normally requires the N-domain of ClpX, which is missing in the PhyB-linker-[ClpX]3 variant.

Baker and Sauer (2009) used this tag to test an artificial tethering system and demonstrated that it can serve as degradation signal for substrates that are tethered to ClpX.


You can visit our wiki "visual description" page for more info


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Conception:

We chose to use the same sequence, which was already successfully used by used by Baker and Sauer (2009): NH2-TNTAKILNFGR. The codon usage of E. coli (http://www.geneinfinity.org/sp_codonusage.html) has been used to decide the DNA sequence, in addition NgoMIV and AgeI restriction sites have been added to the sequence.

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Categories
Parameters
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