Part:BBa_K3647333

Eosinophil cationic protein (N-term. 6XHIS, TEV)
For information and characterization of this part please see: BBa_K3647222

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 40
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

General information

Eosinophil cationic protein (ECP), also known as ribonuclease 3, is a member of the ribonuclease superfamily. Protein is located in the matrix of eosinophil and its release is triggered by an immune stimulus. In humans, ECP is encoded by the RNASE3 gene.(Mastrianni, 1992)

Protein possesses neurotoxic, helmintho-toxic, and has low ribonucleolytic activities. Protein is known to be involved in the antimicrobial humoral response, innate immune response, neutrophil degranulation, RNA catabolic process. (Boix, 2001)

Figure 1. Structure of eosinophil cationic protein. PBD code 1H1H.

Human eosinophil cationic protein is a single-chain, zinc-containing protein with a molecular weight ranging from 16 to 22 kDa. The ECP amino acid sequence has a 32 % identity to human pancreatic ribonuclease (RNase) A. In general, the RNase activity of ECP is required for its neurotoxic and antiviral properties of the protein, but not for its antibacterial and antihelminthic activities.(Kita, 2014)

ECP was shown to be correlated correlates well with airway inflammation in asthma during numerous studies. Serum ECP was found to directly correlate with activated eosinophils in the bronchial mucosa of adult asthmatic patients. In children serum, ECP has also been found to be a more sensitive marker of asthma severity than peripheral blood eosinophil counts in acute exacerbations. (Koh, 2007)