Coding

Part:BBa_K3646006

Designed by: Sukanya Chakraborty   Group: iGEM20_IISER_Berhampur   (2020-10-25)


hSTAT2 CDS

human signal transducer and activation of transcription coding sequence.

The primary component of the innate antiviral response in mammals is interferon (IFN) signalling pathway. A critical component of the human antiviral response is the type 1 interferon (IFN) pathway, which acts to delay virus replication and to stimulate the activation of antiviral effector cells. IFN receptors signal through the Janus kinase-signal transducer and activator of transcription (JAK-STAT) pathway in which JAKs phosphorylate STATs, which then translocate to the nucleus to induce the expression of IFN-stimulated genes (ISGs). Type I IFN signalling activates phosphorylated STAT1 and STAT2 (phosphorylated by Janus kinase 1 (JAK1) and tyrosine kinase 2 (Tyk2), two tyrosine kinases that are associated with IFNAR1/2) to heterodimerize and associate with interferon regulatory factor-9 (IRF9) to form IFN-stimulated gene factor-3 (ISGF3), which specifically binds IFN-stimulated response elements (ISREs) within antiviral gene promoters and triggers transcription and translation of antimicrobial proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2563
    Illegal PstI site found at 25
    Illegal PstI site found at 253
    Illegal PstI site found at 601
    Illegal PstI site found at 1912
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2563
    Illegal PstI site found at 25
    Illegal PstI site found at 253
    Illegal PstI site found at 601
    Illegal PstI site found at 1912
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2563
    Illegal BamHI site found at 303
    Illegal BamHI site found at 449
    Illegal BamHI site found at 1602
    Illegal XhoI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2563
    Illegal PstI site found at 25
    Illegal PstI site found at 253
    Illegal PstI site found at 601
    Illegal PstI site found at 1912
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2563
    Illegal PstI site found at 25
    Illegal PstI site found at 253
    Illegal PstI site found at 601
    Illegal PstI site found at 1912
    Illegal NgoMIV site found at 1766
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2097


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Categories
Parameters
protein