Coding

Part:BBa_K3645008

Designed by: Huang nan   Group: iGEM20_Peking   (2020-10-26)


Mutated TadA from ABE 7.10

Contains the full CDS of ABE 7.10, whose Cas9 part was replace with our lab's dCas9.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2287
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 508
    Illegal BamHI site found at 4566
    Illegal XhoI site found at 882
    Illegal XhoI site found at 1104
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 84
    Illegal AgeI site found at 218
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None