Part:BBa_K364319
Gal4 DBD
This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which encode for the enzymes used to convert galactose to glucose. This protein contains a fungal Zn(2)-Cys(6) binuclear cluster domain.
More on GAL4 DNA binding domain: [http://en.wikipedia.org/wiki/Two-hybrid_screening].
Uniprot GAL4 DNA binding domain: [http://www.uniprot.org/uniprot/P04386].
View GAL4 DNA binding domain crystal structure here: [http://www.rcsb.org/pdb/explore/jmol.do?structureId=3COQ&bionumber=1].
Description of the RXRa DBD crystal structure with reference here:[http://www.cell.com/structure/retrieve/pii/S0969212608001809].
Our Gal4 is flanked by RFC 25 Prefix and Suffix, and thus can be used for assembly of in frame composite parts.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 218
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 137
n/a | Gal4 DBD |