Part:BBa_K364310

EcR LBD

The ecdysone receptor is a nuclear receptor found in arthropods, where it controls development and contributes to other processes such as reproduction. The receptor is a non-covalent heterodimer of two proteins, the EcR protein and ultraspiracle protein (USP). It binds to and is activated by ecdysteroids. Pulses of 20-hydroxyecdysone occur during insect development, whereupon this hormone binds to the ecdysone receptor, a ligand-activated transcription factor found in the nuclei of insect cells. This in turn leads to the activation of many other genes, which ultimately causes physiological changes that result in ecdysis (moulting).

More on Ecdysone receptor here: [http://en.wikipedia.org/wiki/Ecdysone_receptor].

Ecdysone receptor Uniprot: [http://www.uniprot.org/uniprot/P34021].

View the Ecdysone receptor crystal structure here: [http://www.rcsb.org/pdb/explore/jmol.do?structureId=1R1K&bionumber=1].

Picture of Ecdysone receptor USP (Ultraspiracle) heterodimer. Ponasterone A a specific agonist bound to the Ecdysone receptor ligand binding domain.

Description of the RXRa DBD crystal structure with reference here: [http://www.nature.com/nature/journal/v426/n6962/full/nature02112.html].

Picture of gel electrophoresis: EcR LBD in pSB1C3 results a 768 bp long insert

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 683
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]