Composite

Part:BBa_K3635013

Designed by: Emily Blundell   Group: iGEM20_KCL_UK   (2020-10-24)


T7 promoter+RBS+lac operator+Tyrosinase+7xhis-tag+pBluescript II SK+ f1 ori+AmpR

This is the tyrosinase enzyme expression vector which will be used in a co-expression system with Pvfp-5 Beta. It will serve to polymerise the mfp protein in vivo so as to produce a polymer in the E.coli host before purification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 564


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Categories
Parameters
None