Composite

Part:BBa_K3635009

Designed by: Emily Blundell   Group: iGEM20_KCL_UK   (2020-10-24)


T7 promoter+RBS+lac operator+Pvfp-5 Beta7xhis-tag+pBluescript II SK+ f1 ori+AmpR

This is our Pvfp-5 Beta expression Vector. It will produce the beta isoform of the mfp-5 protein under control of the T7 promoter as part of a co-expression system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 564


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Categories
Parameters
None