Reporter
5HTBS

Part:BBa_K3631300

Designed by: Sihan Ju   Group: iGEM20_NJMU-China   (2020-10-23)


Serotonin Whole Cell Biosensor

This composite part is a whole cell biosensor of serotonin. The biosensor plasmid consists two parts: A. tac Promoter + lasR ORF, which serves to express lasR protein; B. lasI Promoter + lasI ORF + linker + lacZ, which serves as a sensor and reporter that will turn the culture medium blue within the presence of serotonin. It would work when transformed into E. coli. This part could be used to detect the presence of serotonin in solutions or human secretions.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1150
    Illegal PstI site found at 157
    Illegal PstI site found at 565
    Illegal PstI site found at 1338
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1150
    Illegal PstI site found at 157
    Illegal PstI site found at 565
    Illegal PstI site found at 1338
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1150
    Illegal BamHI site found at 85
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1150
    Illegal PstI site found at 157
    Illegal PstI site found at 565
    Illegal PstI site found at 1338
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1150
    Illegal PstI site found at 157
    Illegal PstI site found at 565
    Illegal PstI site found at 1338
    Illegal NgoMIV site found at 412
    Illegal NgoMIV site found at 1404
    Illegal AgeI site found at 609
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
biologyEscherichia coli