Part:BBa_K3631100

Part B of Serotonin Whole Cell Biosensor

This part consists a sensor sequence: lasI Promoter, and a fusion protein sequence: lasI ORF + linker + lacZ, which not only serves as a reporter that will turn the culture medium blue within the presence of Qurom-Sensing molecules (QSMs) or proteins alike, but also starts a positive feedback that enhances the transcriptional activity of our responsive promoter. It could be used to design biosensors that aim to detect QSMs and show a visible color change.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 340
  Illegal PstI site found at 528
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 340
  Illegal PstI site found at 528
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 340
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 340
  Illegal PstI site found at 528
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 340
  Illegal PstI site found at 528
  Illegal NgoMIV site found at 594
  
• 1000
  COMPATIBLE WITH RFC[1000]